This collection contains whole ticks collected during tick drag cloth sampling (NEON sample class: tck_identification_in.subsampleID.tck). Tick abundance and diversity are sampled using drag or flag sampling every three weeks at sites where more than five ticks have been detected in the last year and every six weeks elsewhere. Following collection, samples are sent to a professional taxonomist where adult and nymphal ticks are identified to species and lifestage and/or sex, and larvae are enumerated. A portion of identified ticks are then destructively processed for pathogen analysis. Pathogen analysis samples are available through the NEON Biorepository collection titled Mosquito Collection (DNA Extracts [Pathogen Extracts]) (MOSC-PE). The collection outlined here includes tick vouchers preserved in 95% ethanol for final archiving at the U.S. National Tick Collection at Georgia Southern University.