This collection contains genetic extracts from soil microbes collected during terrestrial soil sampling (NEON sample class: mic_dnaExtraction_in.soilDnaSampleID). Three pre-determined, randomly assigned locations are selected for each sampling event within each of 10 plots distributed throughout a site; sampling locations within a plot are not re-sampled. Soil metagenomics sampling occurs annually during the historic peak in vegetation greenness. Soil samples are collected to a maximum depth of 30 cm, with organic and mineral soils sampled separately. Subsamples of homogenized soil (rocks, roots and organic debris removed) from each of the 3 sampling locations are combined to form a plot-level composite sample that is used for metagenomics analysis. Samples are frozen on dry ice and shipped to an analytical facility for DNA extraction, sample preparation and shotgun metagenomic sequencing. Laboratory metadata are then delivered to NEON for QC testing and acceptance, and then are formatted for upload to public sequence repositories. Genetic extracts are shipped from the analytical facility in 96-well plates to the NEON Biorepository to be archived at -80 degrees Celsius. See related links below for protocols and NEON related data products. The metabarcoding protocol used by Battelle Applied Genomics is available in the NEON document library (https://data.neonscience.org/documents).