Surface Water Microbe Collection (DNA Extracts) (NEON-SWMC-DNA)
SAMPLES ARE CURRENTLY UNAVAILABLE. Please contact the NEON Biorepository for information about when these samples will become available. Genetic extracts from Battelle Applied Genomics for shotgun metagenomic sequencing of surface microbes collected during aquatic microbial sampling (NEON sample class: mic_dnaExtraction_in.amcDnaSampleID). Surface water microbes are collected 12 times per year in wadeable streams and 6 times per year in lakes and non-wadeable streams (rivers), at the same time and location as standard recurrent water chemistry samples. Sample timing is based on statistical analysis of environmental parameters. Details on sampling locations and timing are provided in the NEON document Surface Water Chemistry Sampling in Aquatic Habitats. Genetic extracts in 96-well plates from Battelle Applied Genomics are archived in the NEON Biorepository at -80 degrees Celsius. See protocol AOS Protocol and Procedure: Aquatic Microbial Sampling and science design and the NEON Aquatic Sampling Strategy (https://data.neonscience.org/documents) for more information about this sample class. NEON datasets (https://data.neonscience.org/home) associated with this sample class include Surface Water Microbe Cell Count, Surface Water Microbe Group Abundances, Surface Water Microbe Metagenome Sequences, and Surface Water Microbe Marker Gene Sequences. The metabarcoding protocol used by Battelle Applied Genomics is available in the NEON document library (https://data.neonscience.org/documents).