SAMPLES ARE CURRENTLY UNAVAILABLE. Please contact the NEON Biorepository for information about when these samples will become available. Genetic extracts from Battelle Applied Genomics for shotgun metagenomic sequencing of benthic microbes collected during aquatic microbial sampling (NEON sample class: mic_dnaExtraction_in.ambDnaSampleID). Benthic microbes are collected 3 times per year in wadeable streams at the same time and location as algal periphyton samples. Benthic microbes are not collected in lakes and rivers. Benthic biofilms are collected in wadeable streams during periods of stable stream flow using a series of scrubbing procedures, depending on habitat and substratum type. Genetic extracts in 96-well plates from Battelle Applied Genomics are archived in the NEON Biorepository at -80 degrees Celsius. See protocol AOS Protocol and Procedure: Aquatic Microbial Sampling and science design and the NEON Aquatic Sampling Strategy (https://data.neonscience.org/documents) for more information about this sample class. NEON datasets (https://data.neonscience.org/home) associated with this sample class include Benthic Microbe Group Abundances, Benthic Microbe Metagenome Sequences, and Benthic Microbe Marker Gene Sequences. The metabarcoding protocol used by Battelle Applied Genomics is available in the NEON document library (https://data.neonscience.org/documents).