Benthic Microbe Collection (DNA Extracts) (NEON-BEMC-DNA)

This collection contains genetic extracts of benthic microbes collected during aquatic microbial sampling (NEON sample class: mic_dnaExtraction_in.ambDnaSampleID,mic_dnaExtraction_in.ambDnaOnlySampleID). Benthic microbe samples are collected at the same time and location as periphyton (microalgae) samples, three times per year in wadeable streams during aquatic biology bout windows, roughly in spring, summer, and fall. Samples are collected using field-sterile methods using the most appropriate sampler for the habitat and substratum type, including rock scrubs, grab samples, and epiphyton. In wadeable streams, periphyton samples are collected in the two most dominant benthic habitat types (e.g. riffles, runs, pools, step pools). Cobble scrubs are filtered on 0.22 um Sterivex capsule filters, capped and flash-frozen in the field. Grab samples of sediment (silt, sand) or plant material/epiphyton are collected when appropriate and flash frozen in the field. Frozen samples are shipped to an analytical facility for DNA extraction, sample preparation, and shotgun metagenomic sequencing (only for samples collected during the summer collection bout), marker gene sequencing (and subsequent analysis of community composition), and microbial abundance (qPCR). Laboratory metadata are delivered to NEON for QC testing and acceptance, and then are formatted for upload to public sequence repositories. Genetic extracts in 96-well plates are archived at -80 degrees Celsius. Archived extracts range from about 20 to 100 uL. See related links below for protocols and NEON related data products, which include information on the concentration of the DNA. The DNA extraction and sequencing protocols used by Battelle Applied Genomics are available in the NEON document library (https://data.neonscience.org/documents).

"Microbial"Microbial

Contacts: NEON Biorepository, biorepo@asu.edu
Collection Manager: Azhar Husain
Benthic microbe metagenome sequences data product: https://data.neonscience.org/data-products/DP1.20279.001
Benthic microbe marker gene sequences data product: https://data.neonscience.org/data-products/DP1.20280.001
Benthic microbe group abundances data product: https://data.neonscience.org/data-products/DP1.20277.001
Benthic microbe community composition data product: https://data.neonscience.org/data-products/DP1.20086.001
Collection Type: Preserved Specimens
Management: Live Data managed directly within data portal
Global Unique Identifier: bfa83483-221f-4697-a8cc-c56ac1555913
Digital Metadata: EML File
Cite this collection:
Benthic Microbe Collection (DNA Extracts). Occurrence dataset (ID: bfa83483-221f-4697-a8cc-c56ac1555913) https://biorepo.neonscience.org/portal/content/dwca/NEON-BEMC-DNA_DwC-A.zip accessed via the NEON Biorepository Data Portal, https://biorepo.neonscience.org/, 2024-04-15).
Collection Statistics
  • 1,740 specimen records
  • 1,740 (100%) georeferenced
Extra Statistics
Show Geographic Distribution
Show Family Distribution