This collection contains genetic extracts from select fish collected during fish sampling at aquatic sites (NEON sample class: mam_barcoding_in.dnaSubsampleID.fsh). Fish are sampled using a combination of electrofishing, gill-nets and mini-fyke nets in wadeable streams and lakes. Fin clips are collected from 5-10 individuals of a target species. Tissues are preserved in an appropriate tissue vial and shipped to an external lab. DNA is extracted and target sequences amplified via PCR. Barcodes of cytochrome oxidase are generated per specimen. Genetic extracts in 96-well plates from the external lab are archived in the NEON Biorepository at -80 degrees Celsius. See related links below for protocols and NEON related data products. Information about the Canadian Centre for DNA Barcoding (external lab) can be found at https://ccdb.ca/.
NEON Biorepository Data Portal (2022). NEON Biorepository Fish Collection (DNA Extracts). Occurrence dataset https://doi.org/10.15468/qtg4n6 accessed via the NEON Biorepository Data Portal, https://biorepo.neonscience.org/ on 2022-10-06.