Carabid Collection (DNA Extracts) (NEON-CARC-DNA)

This collection contains genetic extracts from the Canadian Centre for DNA Barcoding from select Carabid adults collected during pitfall sampling (NEON sample class: bet_expertTaxonomistID_in.geneticSampleID). Ground beetles are sampled using pitfall traps (16 oz deli containers filled with 150 or 250 mL of propylene glycol). Four traps are deployed in each of 10 plots at each terrestrial NEON site (40 traps per site), with traps arrayed approximately 20 meters from the center of the plot in each of the four cardinal directions. Sampling occurs biweekly throughout the growing season (when temperatures are above 4 degrees C). Up to 10 individuals per species per site for a total of up to 95 individuals per site per year are selected for barcoding. Beetles that are rare, particularly difficult to identify, or poorly represented in previous collection events are prioritized for DNA sequencing. Only beetle specimens that have been identified by an expert taxonomist are eligible for DNA barcoding. Leg tissue is preserved in an appropriate tissue vial and shipped to an external lab, where DNA is extracted and target sequences amplified via PCR. Barcodes of cytochrome oxidase I are generated per specimen. Genetic extracts in 96-well plates from the Canadian Centre for DNA Barcoding are archived in the NEON Biorepository at -80 degrees Celsius. See related links below for protocols and NEON related data products. Information about the Canadian Centre for DNA Barcoding can be found at https://ccdb.ca/.
Contacts: NEON Biorepository (biorepo@asu.edu)
Related link: https://data.neonscience.org/data-products/DP1.10020.001
Collection Type: Preserved Specimens
Management: Live Data managed directly within data portal
Global Unique Identifier: d261934e-0923-446b-9627-95be868627ab
Digital Metadata: EML File
Usage Rights: CC0 1.0 (Public-domain)
Collection Statistics
  • 117 specimen records
  • 0 georeferenced
  • 115 (98%) identified to species
  • 1 families
  • 29 genera
  • 45 species
  • 53 total taxa (including subsp. and var.)
Extra Statistics
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Taxon Distribution
Click on the specimen record counts within the parenthesis to return the records for that family