Carabid Collection (DNA Extracts) (NEON-CARC-DNA)

This collection contains genetic extracts derived from select ground beetle vouchers (Coleoptera: Carabidae; NEON sample class: bet_expertTaxonomistID_in.geneticSampleID). Ground beetles are sampled using pitfall traps (16 oz deli containers filled with 150 or 250 mL of diluted propylene glycol preservative). Multiple traps are deployed in each of 10 plots at each terrestrial NEON site (up to 4 traps per plot; maximum 40 traps per site), with traps arrayed approximately 20 meters from the center of the plot in each of the four cardinal directions. Sampling occurs biweekly throughout the growing season (when temperatures are above 4 degrees C). Following trap collection, all beetles from the family Carabidae are sorted by NEON technicians and identified to species or morphospecies. Annually, up to 95 Carabidae per site are pointed or pinned, receive secondary morphology-based identification by an expert taxonomist, and have genetic sequences produced at the cytochrome oxidase I barcode region. Beetles that are rare, particularly difficult to identify, or poorly represented in previous collection events are prioritized for DNA sequencing. Only beetle specimens that have been identified by an expert taxonomist are eligible for DNA barcoding. Barcodes of cytochrome oxidase I are generated per specimen and are cross-posted on the Barcode of Life Data Portal (http://www.boldsystems.org/). Genetic extracts in 96-well plates from the Canadian Centre for DNA Barcoding are archived in the NEON Biorepository at -80 degrees Celsius. See related links below for protocols and NEON related data products. Information about the Canadian Centre for DNA Barcoding can be found at https://ccdb.ca/.
Contacts: NEON Biorepository, biorepo@asu.edu
Collection Manager: Azhar Husain
Bioinformatician: Laura Rocha Prado, lauraprado@asu.edu (ORCID #: 0000-0003-1237-2824)
NEON BOLD Ground beetle sequences DNA barcode: http://www.boldsystems.org/index.php/Public_SearchTerms?query=BETN
http://www.boldsystems.org/index.php/Public_SearchTerms?query=BETP: NEON BOLD Ground beetle sequences DNA barcode (prototype specimens)
Collection Type: Preserved Specimens
Management: Live Data managed directly within data portal
Global Unique Identifier: d261934e-0923-446b-9627-95be868627ab
Digital Metadata: EML File
GBIF Dataset page: http://www.gbif.org/dataset/44262c91-b3fd-48e4-8e47-1ee03ac2d496
Collection Statistics
  • 9,165 specimen records
  • 9,165 (100%) georeferenced
  • 3 (0.03%) with images (6 total images)
  • 3,483 BOLD genetic references
  • 8,879 (97%) identified to species
  • 2 families
  • 110 genera
  • 549 species
  • 602 total taxa (including subsp. and var.)
Extra Statistics
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