Please contact the NEON Biorepository for information about when these samples will become available. This collection contains genetic extracts from homogenized zooplankton samples collected during zooplankton sampling in lakes (NEON sample class: zoo_dnaExtraction_in.dnaSampleID). Zooplankton DNA metabarcoding samples are collected and sequenced once per year in mid-summer. Samples are collected using either a tow net (water deeper than 4 m) or a Schindler-Patalas trap (water shallower than 4 m) depending on the depth at the sampling location. Samples are collected near the NEON profiling buoy as well as the littoral sensor sets. Zooplankton are preserved in ethanol in the field. The samples are shipped to an external facility for homogenization and high-throughput sequencing (metabarcoding). Genetic extracts in 96-well plates are archived in the NEON Biorepository at -80 degrees Celsius. See related links below for protocols and NEON related data products. The metabarcoding analysis protocol used by Jonah Ventures (external facility) is available in the NEON document library (https://data.neonscience.org/documents). Additional information about Jonah Ventures can be found at https://jonahventures.com/.
NEON Biorepository Data Portal (2023). NEON Biorepository Zooplankton Collection (DNA Extracts).
Occurrence dataset https://doi.org/10.15468/5rmhq9 accessed via the NEON Biorepository Data Portal, https://biorepo.neonscience.org/ on 2023-03-25.