SAMPLES ARE CURRENTLY UNAVAILABLE. Please contact the NEON Biorepository for information about when these samples will become available. This collection contains genetic extracts from homogenized benthic macroinvertbrate samples collected during aquatic macroinvertebrate sampling (NEON sample class: inv_dnaExtraction_in.dnaSampleID). Benthic macroinvertebrate DNA samples are collected three times per year at wadeable stream, river, and lake sites during aquatic biology bout windows, roughly in spring, summer, and fall, at the same time and location as morphological taxonomy samples. Samples are collected using the most appropriate sampler for the habitat type, including Surber, Hess, hand corer, modified kicknet, D-frame sweep, and petite ponar samplers. In wadeable streams, samples are collected in the most dominant habitat type (e.g. riffles, runs, pools, step pools). In lakes and rivers, samples are collected in littoral areas using a D-frame sweep. Samples are preserved in ethanol in the field, returned to the domain support facility for a preservative change prior to shipping to an external facility. The DNA samples from bout 2 (summer) are shipped to a an external facility for homogenization and high-throughput sequencing (metabarcoding). The unused portion of genetic extracts are archived at the NEON Biorepository in 96-well plates and stored at -80 degrees Celsius. See related links below for protocols and NEON related data products. The metabarcoding analysis protocol used by Jonah Ventures (external facility) is available in the NEON document library (https://data.neonscience.org/documents). Additional information about Jonah Ventures can be found at https://jonahventures.com/.