SAMPLES ARE CURRENTLY UNAVAILABLE. Please contact the NEON Biorepository for information about when these samples will become available. This collection contains pathogen extracts from mosquitoes collected during CO2 trapping at terrestrial sites (NEON sample class: mos_pathogenpooling_in.testingVialID). When adult mosquitoes are active, sampling occurs (via CDC light traps) every two weeks at core sites and every four weeks at relocatable sites. A sampling bout consists of two trapping nights and the intervening day for up to ten plots per site. Mosquito legs are collected from up to 10 individuals per species per domain. A set of up to 1000 individual mosquitoes per species per site per year are targeted for pathogen testing of arboviruses within the families Bunyaviridae, Alphaviridae, and Flavivirdae. Mosquito species are prioritized into three tiers, with highest priority given to Tier 1 species (Aedes aegypti and Aedes albopictus); followed by Tier 2 species: Culex tarsalis, Culex pipiens, and Aedes triseriatus; and then Tier 3 species: any other individuals identified to the species-level within the genera of Aedes and Culex. To be eligible for pathogen testing, a species must have a minimum of 100 (Tier 1 species) or 200 (Tiers 2 & 3) individuals present at a site in a year that are available for testing. Mosquitoes that meet pathogen-testing criteria collected within the same site and sampling bout are homogenized into a large pool of female conspecifics and then subdivided into testing vials of appropriate pool sizes for pathogen testing. Each vial is tested one or more times using a variety of methods using a variety of methods which may include RT-PCR, Vero cell culture, and melt curve assays. Pathogen extracts from the University of Massachusetts Laboratory of Medical Zoology are are archived at the NEON Biorepository in 2 mL cryovials and storedat -80 degrees Celsius. See related links below for protocols and NEON related data products.