SAMPLES ARE CURRENTLY UNAVAILABLE. Please contact the NEON Biorepository for information about when these samples will become available. Pathogen extracts from the University of Massachusetts Laboratory of Medical Zoology from mosquitoes collected during CO2 trapping at terrestrial sites (NEON sample class: mos_pathogenpooling_in.testingVialID). Following collection, samples are sent to a professional taxonomist where a subsample of the catch generated from each trap is identified to species and sex. A subset of postively-identified mosquitoes are later processed for pathogen testing. Only female mosquitoes identified to the species-level and captured in sufficient quantity over a season from likely vector species are eligible for pathogen testing. Mosquitoes that meet pathogen-testing criteria collected within the same site and sampling bout are homogenized into a large pool of female conspecifics and then subdivided into testing vials of appropriate pool sizes for pathogen testing. Each vial is tested one or more times using a variety of methods using a variety of methods which may include RT-PCR, Vero cell culture, and melt curve assays. Pathogen extracts from the University of Massachusetts Laboratory of Medical Zoology are are archived at the NEON Biorepository in 2 mL cryovials at -80 degrees Celsius. See TOS Protocol and Procedure: Mosquito Sampling and TOS Science Design for Vectors and Pathogens Vials . NEON datasets (https://data.neonscience.org/home) associated with this sample class include Mosquitoes Sampled from CO2 Traps and Mosquito-borne Pathogen Status. Protocols for pathogen testing are available in the NEON document library (https://data.neonscience.org/documents).