SAMPLES ARE CURRENTLY UNAVAILABLE. Please contact the NEON Biorepository for information about when these samples will become available. Pooled collection of identified benthic macroinvertebrates, approximately 300 individuals subsampled from each habitat (dominant and subdominant) for each sampling event (NEON sample class: inv_fielddata_in.sampleID). This collection does not contain Chironomids or Oligochaetes. Benthic macroinvertebrates are sampled three times per year at at NEON aquatic sites (lakes, rivers and wadeable streams), roughly in spring, summer, and autumn. Benthic macroinvertebrates are sampled using a percent-based macrohabitat approach. Habitats sampled focus on riffles, runs, and pools depending on the percent cover of each habitat within each 1 km-long NEON Aquatic wadeable stream site, and pelagic and littoral samples in lakes and non-wadeable streams (or rivers). Field protocols differ depending on the habitat and substrate being sampled. However, all samples are collected from the surface of the natural substratum in each habitat. Five samples for morphological taxonomy are collected in the dominant habitat type (wadeable stream) or littoral area (lake and river), and three samples for morphological taxonomy are collected in the sub-dominant habitat type (wadeable streams) or pelagic area (lakes and rivers). Subsample vials contain at least 300 identified organisms. Specimens are preserved in 95% ethanol and archived in a temperature (17-18°C) and humidity controlled environment. See AOS Protocol and Procedure: Aquatic Macroinvertebrate Sampling and the NEON Aquatic Sampling Strategy (https://data.neonscience.org/documents) for more information about this sample class. NEON datasets (https://data.neonscience.org/home) associated with this sample class include Macroinvertebrate Collection and Macroinvertebrate DNA Barcode.