Subsample for benthic microbes archive (NEON sample class: amb_fieldParent_in.archiveID). Benthic microbes are collected 3 times per year in wadeable streams at the same time and location as algal periphyton samples. Benthic microbes are not collected in lakes and rivers. Benthic biofilms are collected in wadeable streams during periods of stable stream flow using a series of scrubbing procedures, depending on habitat and substratum type. Microbial samples are collected either as whole sample (plant or sediment grab) or on 0.22 um Sterivex SVGP® L10RC capsule filters for archive and analysis, depending on substratum type at the site. Samples are archived at -80 degrees Celsius. See protocol AOS Protocol and Procedure: Aquatic Microbial Sampling and science design and the NEON Aquatic Sampling Strategy (https://data.neonscience.org/documents) for more information about this sample class. NEON datasets (https://data.neonscience.org/home) associated with this sample class include Benthic microbe group abundances, Benthic microbe metagenome sequences, and Benthic microbe marker gene sequences.