Benthic Microbe Collection (Sterivex Filters) (NEON-BEMC-SF)

This collection contains benthic biofilm samples collected on 67 mm long, 1.7 cm diameter, 0.22 um Sterivex capsule filters (NEON sample class: amb_fieldParent_in.archiveID, amb_fieldParent_in.geneticSampleID). Benthic biofilm samples are collected 3 times per year at the same time and location as periphyton (microalgae) samples and microbe samples sent for sequencing analysis, three times per year in wadeable streams during aquatic biology bout windows, roughly in spring, summer, and fall. Benthic biofilms are not collected in lakes and rivers. Samples are collected from rock and wood scrubs using field-sterile methods, and filtered through a 0.22 um Sterivex SVGP capsule filters. In wadeable streams, periphyton samples are collected in the two most dominant benthic habitat types (e.g. riffles, runs, pools, step pools). Sterivex filters are capped and flash-frozen in the field and then shipped to the Biorepository to be archived at -80 degrees Celsius. See related links below for protocols and NEON related data products.


Contacts: NEON Biorepository,
Collection Manager: Azhar Husain,
Benthic microbe metagenome sequences data product:
Benthic microbe marker gene sequences data product:
Benthic microbe group abundances data product:
Benthic microbe community composition data product:
Collection Type: Preserved Specimens
Management: Live Data managed directly within data portal
Global Unique Identifier: 3818b5a8-60a7-4fef-b57e-8d10d430ecad
Digital Metadata: EML File
EDI Dataset page:
Cite this collection:
NEON Biorepository Data Portal. 2024. Benthic Microbe Collection (Sterivex Filters) (repackaging of occurrences published by the NEON Biorepository Data Portal) Environmental Data Initiative. (Accessed via the NEON Biorepository Data Portal,, 2024-04-24).
Collection Statistics
  • 2,594 specimen records
  • 2,594 (100%) georeferenced
Extra Statistics
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