SAMPLES ARE CURRENTLY UNAVAILABLE. Please contact the NEON Biorepository for information about when these samples will become available. Unsorted bulk benthic macroinvertebrate samples taken in the field (NEON sample class: inv_fielddata_in.geneticSampleID). Benthic macroinvertebrates at NEON aquatic sites (lakes, rivers and wadeable streams) are sampled three times per year at each site, roughly spring, summer, and autumn. Benthic macroinvertebrates are sampled using a percent-based macrohabitat approach. Habitats sampled focus on riffles, runs, and pools depending on the percent cover of each habitat within each 1 km-long NEON Aquatic wadeable stream site, and pelagic and littoral samples in lakes and non-wadeable streams (or rivers). Field protocols differ depending on the habitat and substrate being sampled. However, all samples are collected from the surface of the natural substratum in each habitat. Five samples for morphological taxonomy are collected in the dominant habitat type (wadeable stream) or littoral area (lake and river), and three samples for morphological taxonomy are collected in the sub-dominant habitat type (wadeable streams) or pelagic area (lakes and rivers). An additional three samples for DNA are collected in the dominant habitat type. The DNA samples from bout 2 (summer) are sent to an external laboratory for metabarcoding. The unsorted bulk DNA samples from bout 1 (spring) and bout 3 (autumn) are archived for future research. Unsorted bulk macroinvertebrate samples are preserved in 70-95% ethanol and archived in a temperature (17-18°C) and humidity controlled environment. See AOS Protocol and Procedure: Aquatic Macroinvertebrate Sampling and the NEON Aquatic Sampling Strategy (https://data.neonscience.org/documents) for more information about this sample class. NEON datasets (https://data.neonscience.org/home) associated with this sample class include Macroinvertebrate Collection and Macroinvertebrate DNA Barcode.