Benthic Macroinvertebrate Collection (Slide Mounted Chironomids and Oligochaetes) (NEON-BMIC-MS)

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This collection contains slide-mounted subsamples of chironomids and oligochaetes sorted from bulk benthic macroinvertebrate samples for identification purposes (NEON sample class: inv_pertaxon_in.slideID). Benthic macroinvertebrate samples are collected three times per year at wadeable stream, river, and lake sites during aquatic biology bout windows, roughly in spring, summer, and fall. Samples are collected using the most appropriate sampler for the habitat type, including Surber, Hess, hand corer, modified kicknet, D-frame sweep, and petite ponar samplers. Macroinvertebrates are sampled using a percent-based macrohabitat approach. In wadeable streams, samples are collected in the two most dominant habitat types (e.g. riffles, runs, pools, step pools) within each 1 km-long wadeable stream site. In lakes, samples are collected near the buoy and littoral sensors using a petite ponar, and in littoral areas using a D-frame sweep. In rivers, samples are collected near the buoy and two other deep-water locations using a petite ponar sampler, and in littoral areas using a D-frame sweep or large-woody debris sampler. Field protocols differ depending on the habitat and substrate being sampled, but all samples are collected from the surface of the natural substratum in each habitat. Samples are preserved in ethanol in the field, returned to the domain support facility for a preservative change where a small volume of glycerol is added to help keep invertebrates from becoming brittle. Samples are shipped to a taxonomy lab for sorting and identification, including count of each taxon per size class (to nearest mm) and identification to lowest practical taxon (genus or species). Subsamples are mounted on glass microscope slides and stored at room temperature. See related links below for protocols and NEON related data products.


Contacts: NEON Biorepository,
Collection Manager: Isa Betancourt, (ORCID #: 0000-0003-3873-0693)
Macroinvertebrate collection data product:
Macroinvertebrate metabarcoding data product:
Collection Type: Preserved Specimens
Management: Live Data managed directly within data portal
Global Unique Identifier: 050a9105-3ed1-4785-a3b8-d6cedbccd2a6
Digital Metadata: EML File
GBIF Dataset page:
Cite this collection:
NEON Biorepository Data Portal (2024). NEON Biorepository Benthic Macroinvertebrate Collection (Microscope Slides). Occurrence dataset accessed via the NEON Biorepository Data Portal, on 2024-05-30.
Collection Statistics
  • 532 specimen records
  • 532 (100%) georeferenced
Extra Statistics
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Geographic Distribution
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